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Year : 2019  |  Volume : 3  |  Issue : 2  |  Page : 42-44

Mitotic figures evaluation in oral squamous cell carcinoma using crystal violet and feulgen stains - A comparative study

Department of Oral and Maxillofacial Pathology, Bangalore Institute of Dental Sciences, Bengaluru, Karnataka, India

Correspondence Address:
V Subashini
Department of Oral and Maxillofacial Pathology, Bangalore Institute of Dental Sciences, Bengaluru, Karnataka
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ijofb.ijofb_9_21

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Context: Mitosis is the process of nuclear cell division. Increased mitosis causes excessive proliferation of cells which is observed in oral squamous cell carcinoma (OSCC). However, there is always problem in differentiating a mitotic cell from an apoptotic cell, during routine staining procedures, which affect the reliability of histological grading of OSCC. Recently, several methods have been implemented in identifying these mitotic figures (MFs), but they seem to be time-consuming and expensive which makes them less feasible. Thus, an effort was done to evaluate the efficacy of crystal violet and Feulgen stains in identifying MFs along with routine hematoxylin and eosin (H and E) in OSCC. Aim: In the identification of MFs in diagnosed cases of OSCC using crystal violet and Feulgen stains and H and E stain. Settings and Design: This was a retrospective study. Materials and Methods: The study sample includes five tissue sections of moderately-differentiated and well-differentiated OSCC each and four sections of poorly differentiated which were stained with H and E, Feulgen, and 1% crystal violet stains, and the number of MFs was enumerated. Statistical Analysis Used: One-way ANOVA was used for statistical analysis. Results: The results from our study showed that 1% crystal violet-stained MFs are better than H and E and Feulgen stains. Conclusion: Crystal violet stain can be considered as a simple, reliable, cost-effective, and reproducible method of staining MFs.

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